Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers

Soluble fibrin (SF) in blood consists of monomers lacking both fibrinopeptides A with a minor population in multimeric clusters. It is a substantial component of isolated fibrinogen (fg), which spontaneously self-assembles into protofibrils progressing to fibers at sub-physiologic temperatures, a process enhanced by adsorption to hydrophobic and some metal surfaces. *

In their article “Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers” Dennis K. Galanakis, Anna Protopopova, Kao Li, Yingjie Yu, Tahmeena Ahmed, Lisa Senzel, Ryan Heslin, Mohamed Gouda, Jaseung Koo, John Weisel, Marilyn Manco-Johnson and Miriam Rafailovich mention how they employed topographic and lateral atomic force microscopy (AFM) scanning for imaging fg monomers and soluble polymers. Adsorption to polystyrene (PS) and to trioctylmethylamine (TOMA) coated silica wafers was used.

NANOSENSORS™ SuperSharpSilicon™ SSS-SEIHR high resolution AFM probes (typical AFM tip radius of 2 nm, typical force constant 15 N/m, typical resonance frequency 130 kHz) were used for the atomic force microscopy (AFM) in tapping mode. *

Figure 5 from D. Galanakis et al. “Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers”: AFM images. Horizontal bars denote 100 nm and 50 nm in panels a and b, respectively. a FR fg adsorbed on a TOMA surface showing a large, upper arrow, and a smaller multimeric cluster, lower arrow, showing the marked variation in the cluster size. The smaller cluster also shows regular surface undulations indicating its multimeric composition. b FR fg adsorbed on modified graphite (MG) surface, showing a field of solitary trinocular monomers and two clusters. Inset: a 4 × magnification from a different area of the same field showing a monomer, right arrow, and a multimeric cluster, left arrow NANOSENSORS SuperSharpSilicon SSS-SEIHR AFM probes were used.
Figure 5 from D. Galanakis et al. “Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers”: AFM images. Horizontal bars denote 100 nm and 50 nm in panels a and b, respectively. a FR fg adsorbed on a TOMA surface showing a large, upper arrow, and a smaller multimeric cluster, lower arrow, showing the marked variation in the cluster size. The smaller cluster also shows regular surface undulations indicating its multimeric composition. b FR fg adsorbed on modified graphite (MG) surface, showing a field of solitary trinocular monomers and two clusters. Inset: a 4 × magnification from a different area of the same field showing a monomer, right arrow, and a multimeric cluster, left arrow

*Dennis K. Galanakis, Anna Protopopova, Kao Li, Yingjie Yu, Tahmeena Ahmed, Lisa Senzel, Ryan Heslin, Mohamed Gouda, Jaseung Koo, John Weisel, Marilyn Manco-Johnson and Miriam Rafailovich
Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers
Cell and Tissue Research 387, pages 479–491 (2022)
DOI: https://doi.org/10.1007/s00441-022-03599-9

Please follow this external link to read the full article: https://rdcu.be/cL9qn

Open Access: The article “Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers” by Dennis K. Galanakis, Anna Protopopova, Kao Li, Yingjie Yu, Tahmeena Ahmed, Lisa Senzel, Ryan Heslin, Mohamed Gouda, Jaseung Koo, John Weisel, Marilyn Manco-Johnson and Miriam Rafailovich is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

NANOSENSORS AFM Probes on TV

NANOSENSORS™ PointProbe® Plus AFM probes will have a tiny TV appearance today on BBC 4  “Secrets of Size: Atoms to Supergalaxies – Going Small” presented by  Jim Al-Khalili, directed by Tim Usborne, produced by Paul Sen. Let’s watch it and see if you can spot them among all the other interesting things he is going to present.

The programme will be first broadcast today on BBC 4 at 21:00 p.m. UK time.

Please follow this external link for more detailed information on the programme and further broadcast dates and times https://www.bbc.co.uk/programmes/m0017frm

 

NANOSENSORS™ PointProbe® Plus AFM probe 3D view - research driven excellence since 1990
NANOSENSORS™ PointProbe® Plus AFM probe 3D view

Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures

Discrete block co-oligomers (BCOs) can form highly ordered ultrasmall nanostructures which can be used for lithographic templates. These nanotemplates are promising for the low-cost, large-scale, and high-throughput production of sub-10 nm nanomaterials and nanodevices. However, work-intensive synthetic routes can be an obstacle to their practical application. *

In “Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures” Takuya Isono, Ryoya Komaki, Chaehun Lee, Nao Kawakami, Bian J. Ree, Kodai Watanabe, Kohei Yoshida, Hiroaki Mamiya, Takuya Yamamoto, Redouane Borsali, Kenji Tajima and Toshifumi Satoh report the development of a readily available monodisperse and discrete block co-oligomer (BCO) system consisting of hydrophilic sugars and hydrophobic terpenoids that is capable of forming various self-assembled nanostructures with ultrasmall periodicity.*

The authors believe that the BCOs presented in their publication have the potential to contribute to accelerating applied research of solid and solution state self-assembly of discrete and monodisperse BCOs, thereby expanding their application scopes in various fields of not only the nanolithography but also organic devices, separation materials, coatings, etc.*

NANOSENSORS™ PointProbe® Plus PPP-NCHR standard tapping mode AFM probes and SuperSharpSilicon™  SSS-NCHR high resolution (typical AFM tip radius 2nm)  silicon AFM probes for tapping mode/non-contact mode applications were used for the atomic force microscopy (AFM) phase images presented in the article.

Fig. 4 from : Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures by Takuya Isono et al. Thin-film morphologies of Glc3-b-Sol and Glc4-b-Sol. AFM height images (a, b) and corresponding cross-sectional profiles (c, d) indicating the formation of 6–8-nm-thick horizontal lamellae in Glc3-b-Sol (a, c) and Glc4-b-Sol thin films (b, d). Thin-film samples were prepared by spin-coating the BCO solution onto the hydrophilic surface of a silicon substrate followed by thermal annealing at 85 °C for 1 h. NANOSENSORS PointProbe Plus PPP-NCHR standard silicon tapping mode AFM probes and NANOSENSORS SuperSharpSilicon high resolution silicon AFM probes were used
Fig. 4 from : Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures by Takuya Isono et al.
Thin-film morphologies of Glc3-b-Sol and Glc4-b-Sol.
AFM height images (a, b) and corresponding cross-sectional profiles (c, d) indicating the formation of 6–8-nm-thick horizontal lamellae in Glc3-b-Sol (a, c) and Glc4-b-Sol thin films (b, d). Thin-film samples were prepared by spin-coating the BCO solution onto the hydrophilic surface of a silicon substrate followed by thermal annealing at 85 °C for 1 h.

*Takuya Isono, Ryoya Komaki, Chaehun Lee, Nao Kawakami, Bian J. Ree, Kodai Watanabe, Kohei Yoshida, Hiroaki Mamiya, Takuya Yamamoto, Redouane Borsali, Kenji Tajima and Toshifumi Satoh
Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures
Nature Communications  Chemistry 3, 135 (2020)
DOI: https://doi.org/10.1038/s42004-020-00385-y

Please follow this external link to read the full article: https://rdcu.be/cNeH4

Open Access: The article “Rapid access to discrete and monodisperse block co-oligomers from sugar and terpenoid toward ultrasmall periodic nanostructures” by Takuya Isono, Ryoya Komaki, Chaehun Lee, Nao Kawakami, Bian J. Ree, Kodai Watanabe, Kohei Yoshida, Hiroaki Mamiya, Takuya Yamamoto, Redouane Borsali, Kenji Tajima and Toshifumi Satoh is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.